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Home / ELISA Kit

Human Zinc finger E-box-binding homeobox 2 (ZEB2) ELISA Kit

SKU : BHE13702224

TCF8 encodes a human zinc finger transcription factor that represses T-lymphocyte-specific IL2 gene expression by binding to a negative regulatory domain 100 nucleotides 5-prime of the IL2 transcription start site.
Delta-Ef1 selectively transactivated the promoters of the SMC differentiation marker genes Acta2 and Myh11, and it physically interacted with Srf and Smad3, resulting in synergistic activation of the Acta2 promoter. Chromatin immunoprecipitation assays and knockdown experiments showed that delta-Ef1 was involved in control of SMC differentiation programs induced by Tgf-beta (TGFB1) signaling. Overexpression of delta-Ef1 inhibited neointima formation and promoted SMC differentiation.

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Mfr.No.	KTE60004
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Zinc finger E-box-binding homeobox 2 (ZEB2) ELISA Kit employs a two-site sandwich ELISA to quantitate ZEB2.
Alternative Names	ZEB2; FLJ42816; KIAA0569; SIP-1; SIP1; SMADIP1; ZFHX1B; OTTHUMP00000204542; Smad-interacting protein 1; zinc finger E-box-binding protein 2; zinc finger homeobox 1b
Detection Method	Colorimetric
Kit Components	Human Zinc finger E-box-binding homeobox 2 microplate Human Zinc finger E-box-binding homeobox 2 standard Human Zinc finger E-box-binding homeobox 2 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Zinc finger E-box-binding homeobox 2 (ZEB2) ELISA Kit has high sensitivity and excellent specificity for detection of Human ZEB2. No significant cross-reactivity or interference between Human ZEB2 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	TCF8 encodes a human zinc finger transcription factor that represses T-lymphocyte-specific IL2 gene expression by binding to a negative regulatory domain 100 nucleotides 5-prime of the IL2 transcription start site. Delta-Ef1 selectively transactivated the promoters of the SMC differentiation marker genes Acta2 and Myh11, and it physically interacted with Srf and Smad3, resulting in synergistic activation of the Acta2 promoter. Chromatin immunoprecipitation assays and knockdown experiments showed that delta-Ef1 was involved in control of SMC differentiation programs induced by Tgf-beta (TGFB1) signaling. Overexpression of delta-Ef1 inhibited neointima formation and promoted SMC differentiation.
Notes	This Human Zinc finger E-box-binding homeobox 2 (ZEB2) ELISA Kit employs a two-site sandwich ELISA to quantitate ZEB2 in samples. An antibody specific for ZEB2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyZEB2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ZEB2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ZEB2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	9839

Applications	ELISA
Applications Notes	This Human Zinc finger E-box-binding homeobox 2 (ZEB2) ELISA Kit employs a two-site sandwich ELISA to quantitate ZEB2 in samples. An antibody specific for ZEB2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyZEB2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ZEB2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ZEB2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702224	KTE60004	48 T	$339		Add to Cart
BHE13702224	KTE60004	96 T	$589		Add to Cart
BHE13702224	KTE60004	96 Tx5	$2629		Add to Cart
BHE13702224	KTE60004	96 Tx50	$24569		Add to Cart

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