Human YTHDF3(YTH domain-containing family protein 3) ELISA Kit
Alternative Names
YTH domain-containing family protein 3 ELISA Kit, DF3 ELISA Kit, YTHDF3 ELISA Kit
Reactivity
Human
Detection Method
Double Antibody
Detection Range
31.25-2000pg/ml
Sample Type
Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of YTHDF3 and the recovery rates were calculated by comparing the measured value to the expected amount of YTHDF3 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-104
95
EDTA plasma(n=5)
85-104
96
heparin plasma(n=5)
88-97
92
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of YTHDF3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
93-105%
85-104%
89-105%
EDTA plasma(n=5)
86-97%
82-96%
85-101%
heparin plasma(n=5)
80-96%
83-98%
85-100%
Sensitivity
< 18.75pg/ml
Notes
For Research Use Only
UniProt ID
Q7Z739
Applications
ELISA
Applications Notes
YTHDF3 ELISA Kit allows for the in vitro quantitative determination of YTHDF3 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
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