Matrices listed below were spiked with certain level of YES1 and the recovery rates were calculated by comparing the measured value to the expected amount of YES1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
91-100
96
EDTA plasma(n=5)
86-102
93
heparin plasma(n=5)
86-102
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of YES1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
92-103%
85-104%
93-105%
EDTA plasma(n=5)
86-101%
85-97%
87-97%
heparin plasma(n=5)
88-95%
91-98%
88-100%
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Notes
For Research Use Only
UniProt ID
P07947
Applications
ELISA
Applications Notes
YES1 ELISA Kit allows for the in vitro quantitative determination of YES1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Storage
2-8 °C for 6 months
Reviews of Human YES1 (Tyrosine-protein kinase Yes) ELISA Kit