Human XCL1/Lymphotactin ELISA Kit PicoKine®

Mfr.No.

EK0802

Sensitivity

<1 pg/ml

Detection Range

62.5 pg/ml - 4,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	62.5	125	250	500	1000	2000	4000
O.D.	0.142	0.144	0.146	0.182	0.228	0.372	0.833	1.717

Description

Human XCL1/Lymphotactin ELISA Kit PicoKine® (96 Tests). Quantitate Human XCL1 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Lymphotactin;ATAC;C motif chemokine 1;Cytokine SCM-1;Lymphotaxin;SCM-1-alpha;Small-inducible cytokine C1;XC chemokine ligand 1;XCL1;LTN, SCYC1;

Molecular Weight

12517 MW

Immunogen

Expression system for standard: E.coli; Immunogen sequence: V22-G114

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human XCL1

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	129	466	1208	118	435	1181
Standard deviation	8.51	30.75	54.36	9.2	36.6%3	72.04
CV (%)	6.6%	6.6%	4.5%	7.8%	8.1%	6.1%

Protein Function

Chemotactic activity for lymphocytes but not for monocytes or neutrophils.

Background

Chemokine (C motif) ligand (XCL1) is a small cytokine belonging to the XC chemokine family that is also known as lymphotactin. This gene is mapped to chromosome 1q23 by FISH. The sequence of the deduced 114-amino acid protein is most homologous to the CC chemokines CCL8 and CCL3. It is found in high levels in spleen, thymus, intestine and peripheral blood leukocytes, and at lower levels in lung, prostate gland and ovary. XCL1 induces the migration of cells expressing XCR1.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	129	130	134	146	134	6.75	5%
Sample 2	466	420	445	456	446	17.13	3.8%
Sample 3	1208	1267	1389	1234	1274	69.33	5.4%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	6375
Gene Name	XCL1
UniProt ID	P47992
Protein Name	Lymphotactin
Tissue Specificity	Highest level in spleen, lower in peripheral leukocytes and very low levels in lung, colon and small intestine.
Sequence	Belongs to the intercrine gamma family.

Applications	ELISA
Research Area	Chemokines,Immunology,Innate Immunity

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000624	EK0802	96 wells/kit	$499		Add to Cart

Human XCL1/Lymphotactin ELISA Kit PicoKine®

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