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Home / ELISA Kit

Human WW domain-binding protein 1 (WBP1) ELISA Kit

SKU : BHE13702259

DDOST encodes a component of the oligosaccharyltransferase complex which catalyzes the transfer of high-mannose oligosaccharides to asparagine residues on nascent polypeptides in the lumen of the rough endoplasmic reticulum. The protein complex co-purifies with ribosomes. The product of this gene is also implicated in the processing of advanced glycation endproducts (AGEs), which form from non-enzymatic reactions between sugars and proteins or lipids and are associated with aging and hyperglycemia.DDOST mRNA was expressed intensely in heart and pancreas, but at lower levels in brain. the gene encoding the human DDOST 48-kD subunit is organized into 11 exons spanning about 9 kb. By fluorescence in situ hybridization, they assigned the DDOST subunit gene to chromosome 1p36.1.

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Mfr.No.	KTE60039
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human WW domain-binding protein 1 (WBP1) ELISA Kit employs a two-site sandwich ELISA to quantitate WBP1.
Alternative Names	WBP1; MGC15305; WBP-1;
Detection Method	Colorimetric
Kit Components	Human WW domain-binding protein 1 microplate Human WW domain-binding protein 1 standard Human WW domain-binding protein 1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human WW domain-binding protein 1 (WBP1) ELISA Kit has high sensitivity and excellent specificity for detection of Human WBP1. No significant cross-reactivity or interference between Human WBP1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	DDOST encodes a component of the oligosaccharyltransferase complex which catalyzes the transfer of high-mannose oligosaccharides to asparagine residues on nascent polypeptides in the lumen of the rough endoplasmic reticulum. The protein complex co-purifies with ribosomes. The product of this gene is also implicated in the processing of advanced glycation endproducts (AGEs), which form from non-enzymatic reactions between sugars and proteins or lipids and are associated with aging and hyperglycemia.DDOST mRNA was expressed intensely in heart and pancreas, but at lower levels in brain. the gene encoding the human DDOST 48-kD subunit is organized into 11 exons spanning about 9 kb. By fluorescence in situ hybridization, they assigned the DDOST subunit gene to chromosome 1p36.1.
Notes	This Human WW domain-binding protein 1 (WBP1) ELISA Kit employs a two-site sandwich ELISA to quantitate WBP1 in samples. An antibody specific for WBP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyWBP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for WBP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WBP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	23559

Applications	ELISA
Applications Notes	This Human WW domain-binding protein 1 (WBP1) ELISA Kit employs a two-site sandwich ELISA to quantitate WBP1 in samples. An antibody specific for WBP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyWBP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for WBP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WBP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702259	KTE60039	48 T	$339		Add to Cart
BHE13702259	KTE60039	96 T	$589		Add to Cart
BHE13702259	KTE60039	96 Tx5	$2629		Add to Cart
BHE13702259	KTE60039	96 Tx50	$24569		Add to Cart

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