Human WNT1-inducible-signaling pathway protein 2(WISP2) ELISA kit

Mfr.No.

CSB-EL026120HU

Sensitivity

0.039 ng/mL

Detection Range

0.156 ng/mL-10 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

WNT1 inducible signaling pathway protein 2, CCN5, CT58, CTGF-L, OTTHUMP00000063227, connective tissue growth factor-like protein, wnt-1 signaling pathway protein 2

Recovery

The recovery of human HB-EGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	88	83-92
EDTA plasma (n=4)	97	92-101

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human HB-EGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	89
	Range %	84-93
1:2	Average %	86
	Range %	82-91
1:4	Average %	92
	Range %	85-96
1:8	Average %	105
	Range %	98-109

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
4000	2.214	2.267	2.241	2.141
2000	1.291	1.391	1.341	1.241
1000	0.699	0.745	0.722	0.622
500	0.418	0.412	0.415	0.315
250	0.277	0.284	0.281	0.181
125	0.169	0.175	0.172	0.072
62.5	0.135	0.136	0.136	0.036
0	0.099	0.100	0.100	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	O76076

Applications	ELISA
Research Area	WISP2

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10504868	CSB-EL026120HU	96T	$695	Add to Cart
BHE10504868	CSB-EL026120HU	5x96T	$3336	Add to Cart
BHE10504868	CSB-EL026120HU	10x96T	$6602.50	Add to Cart

Human WNT1-inducible-signaling pathway protein 2(WISP2) ELISA kit

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