Human WNT1-inducible-signaling pathway protein 1(WISP1) ELISA kit

Human WNT1-inducible-signaling pathway protein 1(WISP1) ELISA kit

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Mfr.No.

CSB-EL026119HU

Sensitivity

0.078 ng/mL

Detection Range

0.312 ng/mL-20 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

WNT1 inducible signaling pathway protein 1, CCN4, WISP1c, WISP1i, WISP1tc, WNT1 induced secreted protein 1, Wnt-1 inducible signaling pathway protein 1, wnt-1 signaling pathway protein 1

Recovery

The recovery of human ?2-AP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	83-94
EDTA plasma (n=4)	95	90-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human ?2-AP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	88
	Range %	84-93
1:2	Average %	85
	Range %	81-91
1:4	Average %	92
	Range %	88-100
1:8	Average %	95
	Range %	91-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.272	2.346	2.309	2.214
500	1.476	1.491	1.484	1.389
250	0.863	0.892	0.878	0.783
125	0.448	0.479	0.464	0.369
62.5	0.268	0.251	0.260	0.165
31.2	0.187	0.178	0.183	0.088
15.6	0.169	0.159	0.164	0.069
0	0.097	0.092	0.095	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	O95388

Applications	ELISA
Research Area	WISP1

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10504867	CSB-EL026119HU	96T	$595	Add to Cart
BHE10504867	CSB-EL026119HU	5x96T	$2856	Add to Cart
BHE10504867	CSB-EL026119HU	10x96T	$5652.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.