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Home / ELISA Kit

Human WD repeat-containing protein 52 (WDR52) ELISA Kit

SKU : BHE13702257

WDR53 Contains5 WD repeats. WD-repeat proteins belong to a large and fast-expanding conservative protein family. As demonstrated by the crystal structure of the G protein beta subunit, all WD-repeat proteins are speculated to form a circularized beta propeller structure. The importance of these proteins is not only demonstrated by their critical roles in many essential biological functions ranging from signal transduction, transcription regulation, to apoptosis, but is also recognized by their association with several human diseases. Defining the function of a WD-repeat protein is the current challenge. It is, however, paramount to uncover the function of individual WD-repeat proteins, explore the protein interaction mechanism through WD-repeat domains and, ultimately, understand the complex biological processes and organisms themselves.

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Mfr.No.	KTE60037
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human WD repeat-containing protein 52 (WDR52) ELISA Kit employs a two-site sandwich ELISA to quantitate WDR52.
Alternative Names	WDR52; DKFZp781C082; FLJ11142; MGC126631; MGC142113;
Detection Method	Colorimetric
Kit Components	Human WD repeat-containing protein 52 microplate Human WD repeat-containing protein 52 standard Human WD repeat-containing protein 52 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human WD repeat-containing protein 52 (WDR52) ELISA Kit has high sensitivity and excellent specificity for detection of Human WDR52. No significant cross-reactivity or interference between Human WDR52 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	WDR53 Contains5 WD repeats. WD-repeat proteins belong to a large and fast-expanding conservative protein family. As demonstrated by the crystal structure of the G protein beta subunit, all WD-repeat proteins are speculated to form a circularized beta propeller structure. The importance of these proteins is not only demonstrated by their critical roles in many essential biological functions ranging from signal transduction, transcription regulation, to apoptosis, but is also recognized by their association with several human diseases. Defining the function of a WD-repeat protein is the current challenge. It is, however, paramount to uncover the function of individual WD-repeat proteins, explore the protein interaction mechanism through WD-repeat domains and, ultimately, understand the complex biological processes and organisms themselves.
Notes	This Human WD repeat-containing protein 52 (WDR52) ELISA Kit employs a two-site sandwich ELISA to quantitate WDR52 in samples. An antibody specific for WDR52 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyWDR52 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for WDR52 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WDR52 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	55779

Applications	ELISA
Applications Notes	This Human WD repeat-containing protein 52 (WDR52) ELISA Kit employs a two-site sandwich ELISA to quantitate WDR52 in samples. An antibody specific for WDR52 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyWDR52 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for WDR52 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WDR52 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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