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Home / ELISA Kit

Human WD repeat-containing protein 35 (WDR35) ELISA Kit

SKU : BHE13702258

WDR35 is WD40 domain-containing protein. Studies in mouse and several lower organisms have implicated WDR35 in intraflagellar transport. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. The deduced 1,170-amino acid protein contains 4 N-terminal WD40 domains and shares 92.7% identity with human WDR35. Quantitative RT-PCR detected Wdr35 expression in all rat tissues examined, with highest expression in testis, followed by brain, and lowest expression in spleen.

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Mfr.No.	KTE60038
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human WD repeat-containing protein 35 (WDR35) ELISA Kit employs a two-site sandwich ELISA to quantitate WDR35.
Alternative Names	WDR35; KIAA1336; MGC33196;
Detection Method	Colorimetric
Kit Components	Human WD repeat-containing protein 35 microplate Human WD repeat-containing protein 35 standard Human WD repeat-containing protein 35 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human WD repeat-containing protein 35 (WDR35) ELISA Kit has high sensitivity and excellent specificity for detection of Human WDR35. No significant cross-reactivity or interference between Human WDR35 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	WDR35 is WD40 domain-containing protein. Studies in mouse and several lower organisms have implicated WDR35 in intraflagellar transport. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. The deduced 1,170-amino acid protein contains 4 N-terminal WD40 domains and shares 92.7% identity with human WDR35. Quantitative RT-PCR detected Wdr35 expression in all rat tissues examined, with highest expression in testis, followed by brain, and lowest expression in spleen.
Notes	This Human WD repeat-containing protein 35 (WDR35) ELISA Kit employs a two-site sandwich ELISA to quantitate WDR35 in samples. An antibody specific for WDR35 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyWDR35 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for WDR35 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WDR35 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	57539

Applications	ELISA
Applications Notes	This Human WD repeat-containing protein 35 (WDR35) ELISA Kit employs a two-site sandwich ELISA to quantitate WDR35 in samples. An antibody specific for WDR35 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyWDR35 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for WDR35 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WDR35 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702258	KTE60038	48 T	$339		Add to Cart
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