Human Wd Repeat- and Fyve Domain-containing Protein 4

Mfr.No.

E6408Hu

Alternative Names

C10orf64;WD repeat- and FYVE domain-containing protein 4;WDFY4

Reactivity

Human

Detection Range

10-2000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

353610

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

5.39ng/L

Background

Plays a critical role in the regulation of cDC1-mediated cross-presentation of viral and tumor antigens in dendritic cells. Mechanistically, acts near the plasma membrane and interacts with endosomal membranes to promote endosomal-to-cytosol antigen trafficking. Plays also a role in B-cell survival through regulation of autophagy.

Gene ID	57705
UniProt ID	Q6ZS81
Antigen	WDFY4

Research Area

Cell Biology

Protocol

These standard curves of E6408Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200ng/L	2.507	2.431	2.375
	2.354
600ng/L	1.393	1.391	1.336
	1.389
300ng/L	0.839	0.85	0.795
	0.861
150ng/L	0.374	0.392	0.337
	0.41
75ng/L	0.243	0.231	0.176
	0.22
0ng/L	0.066	0.055	0
	0.044

Precision

We measured random samples of E6408Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	201.37	10.8	5.37
Intra-Assay	2	18	466.88	26.25	5.62
Intra-Assay	3	18	595.48	22.62	3.8

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12107770	E6408Hu	48T	$320		Add to Cart
BHE12107770	E6408Hu	96T	$458		Add to Cart

Human Wd Repeat- and Fyve Domain-containing Protein 4 - WDFY4 ELISA Kit

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