Human von Willebrand Factor cleaving protease,ADAMTS-13/vWF-cp ELISA Kit

Human von Willebrand Factor cleaving protease,ADAMTS-13/vWF-cp ELISA Kit

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Mfr.No.

CSB-E13487h

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

ADAM metallopeptidase with thrombospondin type 1 motif, 13, C9orf8, DKFZp434C2322, FLJ42993, MGC118899, MGC118900, TTP, VWFCP, vWF-CP, a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 13, vWF-cleaving protease, von Wil

Recovery

The recovery of mouse P-Selectin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	92-99
EDTA plasma (n=4)	97	95-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse P-Selectin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:100	Average %	92
	Range %	84-98
1:200	Average %	95
	Range %	81-101
1:400	Average %	97
	Range %	93-103
1:800	Average %	95
	Range %	89-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.768	2.710	2.739	2.568
1000	2.263	2.224	2.244	2.073
500	1.576	1.557	1.567	1.396
250	1.026	1.004	1.015	0.844
125	0.595	0.611	0.603	0.432
62.5	0.441	0.438	0.440	0.269
31.25	0.289	0.271	0.280	0.109
0	0.169	0.172	0.171	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

UniProt ID	Q76LX8

Applications	ELISA
Research Area	ADAMTS13

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10504856	CSB-E13487h	96T	$595	Add to Cart
BHE10504856	CSB-E13487h	5x96T	$2856	Add to Cart
BHE10504856	CSB-E13487h	10x96T	$5652.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.