JavaScript is currently not supported/disabled by this browser. Please enable JavaScript for full functionality.

Call Us 1 - 866 - 986 - 9598
[email protected]

Sign Up

Login
- My Order
- Return Requests
- Track my Order

Home / ELISA Kit

Human Von Willebrand factor A domain-containing protein 3A (VWA3A) ELISA Kit

SKU : BHE13702263

vWA3A,Contains 2 VWFA domains, Human WARP shares 79% amino acid identity with mouse Warp. RT-PCR of mRNA from various mouse tissues and cell lines detected Warp expression only in chondrocytes. Northern blot analysis confirmed expression of Warp in chondrocytes isolated from newborn mouse rib cartilage.
Western blot analysis of transfected human embryonic kidney cells detected a 48-kD protein in both cell layer and media fractions. The majority of the protein was detected in the medium, indicating that WARP is efficiently secreted. Under nonreducing conditions, a 102-kD form was detected, suggesting that WARP forms a disulfide-bonded homodimer. N-glycosidase digestion led to a mobility shift from 48 to 45 kD, indicating that WARP has 1 or more N-linked oligosaccharide chains.

Continuing the saving: $50 Off All ELISA Kits Offer Extended!

Contact us to order

Tel

+1 866.986.9598

Email

[email protected]

Get a Quote

Request More Infos

Abbkine Scientific Co., Ltd.

view supplier details

Order Now

Credit card payments now incur a 3% fee.

Contact us to order

Tel

+1 866.986.9598

Email

[email protected]

Get a Quote

Ask Merchant

Request More Infos

Sample Request

Mfr.No.	KTE60043
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Von Willebrand factor A domain-containing protein 3A (VWA3A) ELISA Kit employs a two-site sandwich ELISA to quantitate VWA3A.
Alternative Names	VWA3A; hCG_2039627; FLJ40941; FLJ46765;
Detection Method	Colorimetric
Kit Components	Human Von Willebrand factor A domain-containing protein 3A microplate Human Von Willebrand factor A domain-containing protein 3A standard Human Von Willebrand factor A domain-containing protein 3A detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Von Willebrand factor A domain-containing protein 3A (VWA3A) ELISA Kit has high sensitivity and excellent specificity for detection of Human VWA3A. No significant cross-reactivity or interference between Human VWA3A and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	vWA3A,Contains 2 VWFA domains, Human WARP shares 79% amino acid identity with mouse Warp. RT-PCR of mRNA from various mouse tissues and cell lines detected Warp expression only in chondrocytes. Northern blot analysis confirmed expression of Warp in chondrocytes isolated from newborn mouse rib cartilage. Western blot analysis of transfected human embryonic kidney cells detected a 48-kD protein in both cell layer and media fractions. The majority of the protein was detected in the medium, indicating that WARP is efficiently secreted. Under nonreducing conditions, a 102-kD form was detected, suggesting that WARP forms a disulfide-bonded homodimer. N-glycosidase digestion led to a mobility shift from 48 to 45 kD, indicating that WARP has 1 or more N-linked oligosaccharide chains.
Notes	This Human Von Willebrand factor A domain-containing protein 3A (VWA3A) ELISA Kit employs a two-site sandwich ELISA to quantitate VWA3A in samples. An antibody specific for VWA3A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyVWA3A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VWA3A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VWA3A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	146177

Applications	ELISA
Applications Notes	This Human Von Willebrand factor A domain-containing protein 3A (VWA3A) ELISA Kit employs a two-site sandwich ELISA to quantitate VWA3A in samples. An antibody specific for VWA3A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyVWA3A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VWA3A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VWA3A bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

No Description Available.

Have you used this product?

Rate it now..!!

Login to Review

SKU	Supplier No.	Size	Your price	Quantity
BHE13702263	KTE60043	48 T	$339		Add to Cart
BHE13702263	KTE60043	96 T	$589		Add to Cart
BHE13702263	KTE60043	96 Tx5	$2629		Add to Cart
BHE13702263	KTE60043	96 Tx50	$24569		Add to Cart

ebiohippo.com provides biomedical researchers with a one-stop informatic shopping experience by transforming biotechnology products’ data into a ready-to-analyze state. We collect and unify suppliers’ data on a single platform to reduce the time of searching, uncertainty, and cost of scientific experiments.

© 2024 Biohippo. All rights reserved.