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Home / ELISA Kit

Human Voltage-dependent anion-selective channel protein 1 (VDAC1) ELISA Kit

SKU : BHE13702281

The voltage-dependent anion channel (VDAC) of the outer mitochondrial membrane is a small, abundant outer membrane pore-forming protein found in the outer membranes of all eukaryotic mitochondria. The VDAC protein is thought to form the major pathway for movement of adenine nucleotides through the outer membrane and to be the mitochondrial binding site for hexokinase and glycerol kinase.
Mitochondria expressing VDAC1 were capable of specifically binding hexokinase, whereas mitochondria expressing VDAC2 only bound hexokinase at background levels. Each human cDNA was expressed in essentially all human cell lines and tissues examined.

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Mfr.No.	KTE60062
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Voltage-dependent anion-selective channel protein 1 (VDAC1) ELISA Kit employs a two-site sandwich ELISA to quantitate VDAC1.
Alternative Names	VDAC1; MGC111064; PORIN; PORIN-31-HL; OTTHUMP00000159381; OTTHUMP00000165946
Detection Method	Colorimetric
Kit Components	Human Voltage-dependent anion-selective channel protein 1 microplate Human Voltage-dependent anion-selective channel protein 1 standard Human Voltage-dependent anion-selective channel protein 1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Voltage-dependent anion-selective channel protein 1 (VDAC1) ELISA Kit has high sensitivity and excellent specificity for detection of Human VDAC1. No significant cross-reactivity or interference between Human VDAC1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	The voltage-dependent anion channel (VDAC) of the outer mitochondrial membrane is a small, abundant outer membrane pore-forming protein found in the outer membranes of all eukaryotic mitochondria. The VDAC protein is thought to form the major pathway for movement of adenine nucleotides through the outer membrane and to be the mitochondrial binding site for hexokinase and glycerol kinase. Mitochondria expressing VDAC1 were capable of specifically binding hexokinase, whereas mitochondria expressing VDAC2 only bound hexokinase at background levels. Each human cDNA was expressed in essentially all human cell lines and tissues examined.
Notes	This Human Voltage-dependent anion-selective channel protein 1 (VDAC1) ELISA Kit employs a two-site sandwich ELISA to quantitate VDAC1 in samples. An antibody specific for VDAC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyVDAC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VDAC1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VDAC1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	7416

Applications	ELISA
Applications Notes	This Human Voltage-dependent anion-selective channel protein 1 (VDAC1) ELISA Kit employs a two-site sandwich ELISA to quantitate VDAC1 in samples. An antibody specific for VDAC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyVDAC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VDAC1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VDAC1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702281	KTE60062	48 T	$339		Add to Cart
BHE13702281	KTE60062	96 T	$589		Add to Cart
BHE13702281	KTE60062	96 Tx5	$2629		Add to Cart
BHE13702281	KTE60062	96 Tx50	$24569		Add to Cart

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