Human Vitronectin - VTN ELISA Kit

Mfr.No.

E0284Hu

Sensitivity

5.12ng/L

Detection Range

10-3000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Vitronectin (also known as VNR) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Serum-spreading factor;Somatomedin-B);S-protein;V75;Vitronectin;Vitronectin V10 subunit;Vitronectin V65 subunit;VN;VNT;VTN

Molecular Weight

54306

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0284Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1045.03	53.54	5.12
Intra-Assay	2	18	297.56	19.18	6.44
Intra-Assay	3	18	207.1	17.98	8.68

Background

Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway. Somatomedin-B is a growth hormone-dependent serum factor with protease-inhibiting activity.

Protocol

These standard curves of E0284Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.137	2.156	2.123
	2.175
800ng/L	1.562	1.542	1.509
	1.522
400ng/L	1.059	1.017	0.984
	0.975
200ng/L	0.687	0.717	0.684
	0.747
100ng/L	0.427	0.434	0.401
	0.44
0ng/L	0.028	0.033	0
	0.038

Gene ID	7448
UniProt ID	P04004

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12101995	E0284Hu	48T	$320		Add to Cart
BHE12101995	E0284Hu	96T	$458		Add to Cart

Human Vitronectin - VTN ELISA Kit

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