Human Vitamin D-binding protein,DBP ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E11859h

Sensitivity

0.078 ng/ml

Detection Range

0.3125 ng/ml - 20ng/ml

Reactivity

Human

Assay Duration

1-5h

Assay Type

Competitive

Recovery

The recovery of human POMC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	85-96
EDTA plasma (n=4)	102	98-107

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human POMC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	96
	Range %	90-102
1:2	Average %	89
	Range %	84-95
1:4	Average %	95
	Range %	90-100
1:8	Average %	92
	Range %	87-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

UniProt ID	P02774

Applications	ELISA
Research Area	GC

Storage	Store at 2-8°C. Please refer to protocol.

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