Human Vitamin D 25-hydroxylase(CYP2R1) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-EL006431HU

Sensitivity

3.9 pg/mL

Detection Range

15.6 pg/mL-1000 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

cytochrome P450, family 2, subfamily R, polypeptide 1, MGC4663, cytochrome P450, family 2, R1, vitamin D 25-hydroxylase

Recovery

The recovery of mouse BNP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	90-95
EDTA plasma (n=4)	103	101-106

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse BNP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	86-98
1:2	Average %	96
	Range %	90-102
1:4	Average %	103
	Range %	100-107
1:8	Average %	89
	Range %	84-95

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q6VVX0

Applications	ELISA
Research Area	CYP2R1

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10504844	CSB-EL006431HU	96T	$695	Add to Cart
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