Human visfatin ELISA Kit

Human visfatin ELISA Kit

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Mfr.No.

CSB-E08940h

Sensitivity

0.156 ng/mL

Detection Range

0.625 ng/mL-40 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

nicotinamide phosphoribosyltransferase, 1110035O14Rik, DKFZp666B131, MGC117256, PBEF, PBEF1, VF, VISFATIN, NAmPRTase, pre-B cell-enhancing factor, pre-B-cell colony enhancing factor 1

Recovery

The recovery of human cath-D spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	89-96
EDTA plasma (n=4)	95	91-101

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human cath-D in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	92
	Range %	85-98
1:2	Average %	96
	Range %	88-100
1:4	Average %	95
	Range %	89-100
1:8	Average %	97
	Range %	91-101

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.004	1.997	2.001	1.953
1000	1.321	1.356	1.339	1.291
500	0.785	0.821	0.803	0.755
167	0.364	0.341	0.353	0.305
83	0.216	0.229	0.223	0.175
0	0.047	0.048	0.048	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P43490

Applications	ELISA
Research Area	NAMPT

Storage	Store at 2-8°C. Please refer to protocol.

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BHE10504841	CSB-E08940h	10x96T	$7505	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<10%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.