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Home / ELISA Kit

Human Villin-1 (VIL1) ELISA Kit

SKU : BHE13702275

Villin is a 92.5 kDa tissue-specific actin-binding protein associated with the actin core bundle of the brush border. Villin contains multiple gelsolin-like domains capped by a small (8.5 kDa) "headpiece" at the C-terminus consisting of a fast and independently-folding three-helix bundle that is stabilized by hydrophobic interactions. The headpiece domain is a commonly studied protein in molecular dynamics due to its small size and fast folding kinetics and short primary sequence.Villin is an actin binding protein expressed mainly in the brush border of the epithelium in vertebrates but sometimes it is ubiquitously expressed in protists and plants. Villin is found localized in the microvilli of the brush border of the epithelium lining of the gut and renal tubes in vertebrates.

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Mfr.No.	KTE60055
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Villin-1 (VIL1) ELISA Kit employs a two-site sandwich ELISA to quantitate VIL1.
Alternative Names	VIL1; D2S1471; VIL;
Detection Method	Colorimetric
Kit Components	Human Villin-1 microplate Human Villin-1 standard Human Villin-1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Villin-1 (VIL1) ELISA Kit has high sensitivity and excellent specificity for detection of Human VIL1. No significant cross-reactivity or interference between Human VIL1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Villin is a 92.5 kDa tissue-specific actin-binding protein associated with the actin core bundle of the brush border. Villin contains multiple gelsolin-like domains capped by a small (8.5 kDa) "headpiece" at the C-terminus consisting of a fast and independently-folding three-helix bundle that is stabilized by hydrophobic interactions. The headpiece domain is a commonly studied protein in molecular dynamics due to its small size and fast folding kinetics and short primary sequence.Villin is an actin binding protein expressed mainly in the brush border of the epithelium in vertebrates but sometimes it is ubiquitously expressed in protists and plants. Villin is found localized in the microvilli of the brush border of the epithelium lining of the gut and renal tubes in vertebrates.
Notes	This Human Villin-1 (VIL1) ELISA Kit employs a two-site sandwich ELISA to quantitate VIL1 in samples. An antibody specific for VIL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyVIL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VIL1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VIL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	7429

Applications	ELISA
Applications Notes	This Human Villin-1 (VIL1) ELISA Kit employs a two-site sandwich ELISA to quantitate VIL1 in samples. An antibody specific for VIL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyVIL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VIL1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VIL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702275	KTE60055	48 T	$339		Add to Cart
BHE13702275	KTE60055	96 T	$589		Add to Cart
BHE13702275	KTE60055	96 Tx5	$2629		Add to Cart
BHE13702275	KTE60055	96 Tx50	$24569		Add to Cart

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