Human Vesicle-associated Membrane Protein-associated Protein A

Mfr.No.

E6397Hu

Sensitivity

8.91ng/L

Detection Range

20-4500ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

33 kDa VAMP-associated protein;hVAP-33;VAMP-A;VAMP-associated protein A;VAP33;VAP-33;VAPA;VAP-A;Vesicle-associated membrane protein-associated protein A;

Molecular Weight

27893

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E6397Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	584.67	44.59	7.63
Intra-Assay	2	18	963.37	35.47	3.68
Intra-Assay	3	18	257.83	19.24	7.46

Background

Binds to OSBPL3, which mediates recruitment of VAPA to plasma membrane sites (PubMed:25447204). The ORP3-VAPA complex stimulates RRAS signaling which in turn attenuates integrin beta-1 (ITGB1) activation at the cell surface (PubMed:25447204). With OSBPL3, may regulate ER morphology (PubMed:16143324). May play a role in vesicle trafficking (PubMed:11511104, PubMed:19289470).

Protocol

These standard curves of E6397Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	2.203	2.236	2.191
	2.268
1200ng/L	1.582	1.556	1.511
	1.529
600ng/L	1.11	1.074	1.029
	1.038
300ng/L	0.674	0.673	0.629
	0.673
150ng/L	0.4	0.424	0.379
	0.448
0ng/L	0.046	0.045	0
	0.043

Gene ID	9218
UniProt ID	Q9P0L0

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12107759	E6397Hu	48T	$320		Add to Cart
BHE12107759	E6397Hu	96T	$458		Add to Cart

Human Vesicle-associated Membrane Protein-associated Protein A - VAPA ELISA Kit

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