Human very low density lipoprotein(VLDL)ELISA Kit

Mfr.No.

CSB-E17087h

Alternative Names

very low density lipoprotein(VLDL)

Reactivity

Human

Detection Range

0.78 ug/mL-50 ug/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Competitive

Assay Duration

1-5h

Recovery

The recovery of human SERPINE2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	88	84-92
EDTA plasma (n=4)	107	103-110

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human SERPINE2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	85
	Range %	82-87
1:2	Average %	96
	Range %	93-99
1:4	Average %	98
	Range %	94-103
1:8	Average %	97
	Range %	93-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.281	2.108	2.195	2.065
10	1.405	1.432	1.419	1.289
5	0.822	0.801	0.812	0.682
2.5	0.483	0.468	0.476	0.346
1.25	0.365	0.372	0.369	0.239
0.625	0.287	0.298	0.293	0.163
0.312	0.222	0.220	0.221	0.091
0	0.131	0.129	0.130	?

Species

Homo sapiens

Sensitivity

0.195 ug/mL

Limit Of Detection

450 nm

Antigen	very low density lipoprotein(VLDL)

Applications

ELISA

Research Area

VLDL

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

Reference	A Preliminary Comparative Assessment of Apelin Serum Levels in Persistent Atrial Fibrillation and Coronary Artery Disease Elnaz Khorrami.et al,/,May In vitro effects of active components of Polygonum Multiflorum Radix on enzymes involved in the lipid metabolism Wang W et al,J Ethnopharmacol,2014 Mar 26.pii: S0378-8741(14)00232-3. The adverse effects of long-term l-carnitine supplementation on liver and kidney function in rats Liu L et al,Hum Exp Toxicol,2015 Feb 9.pii: 0960327115571767.

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SKU	Supplier No.	Size	Your price
BHE10504830	CSB-E17087h	96T	$595	Add to Cart
BHE10504830	CSB-E17087h	5x96T	$2856	Add to Cart
BHE10504830	CSB-E17087h	10x96T	$5652.50	Add to Cart

Human very low density lipoprotein(VLDL)ELISA Kit

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