Human versican/PG-M/PG-350 ELISA kit

Mfr.No.

CSB-E11884h

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

versican, CSPG2, DKFZp686K06110, ERVR, GHAP, PG-M, WGN, WGN1, chondroitin sulfate proteoglycan 2, glial hyaluronate-binding protein, versican proteoglycan

Recovery

The recovery of human PMCH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	100	95-104
EDTA plasma (n=4)	84	80-88

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human PMCH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	95
	Range %	90-100
1:2	Average %	87
	Range %	83-91
1:4	Average %	92
	Range %	88-96
1:8	Average %	90
	Range %	85-94

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.682	2.533	2.608	2.400
500	2.040	1.946	1.993	1.785
250	1.419	1.357	1.388	1.180
125	0.927	0.975	0.951	0.743
62.5	0.654	0.689	0.672	0.464
31.2	0.408	0.392	0.400	0.192
15.6	0.335	0.324	0.330	0.122
0	0.204	0.211	0.208	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

UniProt ID	P13611

Applications	ELISA
Research Area	VCAN

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10504828	CSB-E11884h	96T	$695	Add to Cart
BHE10504828	CSB-E11884h	5x96T	$3336	Add to Cart
BHE10504828	CSB-E11884h	10x96T	$6602.50	Add to Cart

Human versican/PG-M/PG-350 ELISA kit

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