Human Vasohibin-1(VASH1) ELISA kit

Mfr.No.

CSB-EL025794HU

Alternative Names

vasohibin 1, KIAA1036

Reactivity

Human

Detection Range

62.5 pg/mL-4000 pg/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of rat HSP-27 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	85-93
EDTA plasma (n=4)	85	80-90

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat HSP-27 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	98
	Range %	93-102
1:2	Average %	105
	Range %	100-113
1:4	Average %	91
	Range %	85-96
1:8	Average %	94
	Range %	90-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Sensitivity

15.6 pg/mL

Limit Of Detection

450 nm

UniProt ID	Q7L8A9
Antigen	Vasohibin 1

Applications

ELISA

Research Area

VASH1

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

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