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Home / ELISA Kit

Human Vasoactive Intestinal Peptide (VIP) ELISA Kit

SKU : BHE13704868

Vasoactive intestinal peptide (VIP) is a peptide hormone containing 28 amino acid residues and is produced in many areas of the human body including the gut, pancreas and suprachiasmatic nuclei of the hypothalamus in the brain.It has a half-life in the blood of about two minutes. With respect to the digestive system, VIP seems to induce smooth muscle relaxation (lower esophageal sphincter, stomach, gallbladder), stimulate secretion of water into pancreatic juice and bile, and cause inhibition of gastric acid secretion and absorption from the intestinal lumen. Its role in the intestine is to greatly stimulate secretion of water and electrolytes, as well as dilating intestinal smooth muscle, dilating peripheral blood vessels, stimulating pancreatic bicarbonate secretion, and inhibiting gastrin-stimulated gastric acid secretion.

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Mfr.No.	KTE62665
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Vasoactive Intestinal Peptide (VIP) ELISA Kit employs a two-site sandwich ELISA to quantitate VIP.
Alternative Names	VIP; MGC13587; PHM27;
Detection Method	Colorimetric
Kit Components	Human Vasoactive Intestinal Peptide microplate Human Vasoactive Intestinal Peptide standard Human Vasoactive Intestinal Peptide detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Vasoactive Intestinal Peptide (VIP) ELISA Kit has high sensitivity and excellent specificity for detection of Human VIP. No significant cross-reactivity or interference between Human VIP and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Vasoactive intestinal peptide (VIP) is a peptide hormone containing 28 amino acid residues and is produced in many areas of the human body including the gut, pancreas and suprachiasmatic nuclei of the hypothalamus in the brain.It has a half-life in the blood of about two minutes. With respect to the digestive system, VIP seems to induce smooth muscle relaxation (lower esophageal sphincter, stomach, gallbladder), stimulate secretion of water into pancreatic juice and bile, and cause inhibition of gastric acid secretion and absorption from the intestinal lumen. Its role in the intestine is to greatly stimulate secretion of water and electrolytes, as well as dilating intestinal smooth muscle, dilating peripheral blood vessels, stimulating pancreatic bicarbonate secretion, and inhibiting gastrin-stimulated gastric acid secretion.
Notes	This Human Vasoactive Intestinal Peptide (VIP) ELISA Kit employs a two-site sandwich ELISA to quantitate VIP in samples. An antibody specific for VIP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyVIP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VIP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VIP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	7432

Applications	ELISA
Applications Notes	This Human Vasoactive Intestinal Peptide (VIP) ELISA Kit employs a two-site sandwich ELISA to quantitate VIP in samples. An antibody specific for VIP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyVIP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VIP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VIP bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13704868	KTE62665	48 T	$359		Add to Cart
BHE13704868	KTE62665	96 T	$619		Add to Cart
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BHE13704868	KTE62665	96 Tx50	$26209		Add to Cart

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