Matrices listed below were spiked with certain level of Vasn and the recovery rates were calculated by comparing the measured value to the expected amount of Vasn in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
93-104
98
EDTA plasma(n=5)
95-105
100
heparin plasma(n=5)
90-101
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Vasn and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
94-105%
86-105%
90-105%
EDTA plasma(n=5)
90-96%
89-100%
89-97%
heparin plasma(n=5)
90-100%
94-100%
91-100%
Sensitivity
< 9.375pg/ml
Notes
For Research Use Only
UniProt ID
Q6EMK4
Applications
ELISA
Applications Notes
Vasn ELISA Kit allows for the in vitro quantitative determination of Vasn concentrations in serum, plasma, tissue homogenates and other biological fluids.