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Home / ELISA Kit

Human Vascular endothelial cell growth factor receptor 2 (VEGFR-2/Flk-1) ELISA Kit

SKU : BHE13704140

Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. KDR encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc.. Mutations of KDR are implicated in infantile capillary hemangiomas.

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Mfr.No.	KTE61925
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Vascular endothelial cell growth factor receptor 2 (VEGFR-2/Flk-1) ELISA Kit employs a two-site sandwich ELISA to quantitate KDR.
Alternative Names	KDR; CD309; FLK1; VEGFR; VEGFR2; fetal liver kinase-1; kinase insert domain receptor; protein-tyrosine kinase receptor Flk-1; soluble VEGFR2; tyrosine kinase growth factor receptor; vascular endothelial growt
Detection Method	Colorimetric
Kit Components	Human Vascular endothelial cell growth factor receptor 2 microplate Human Vascular endothelial cell growth factor receptor 2 standard Human Vascular endothelial cell growth factor receptor 2 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Vascular endothelial cell growth factor receptor 2 (VEGFR-2/Flk-1) ELISA Kit has high sensitivity and excellent specificity for detection of Human KDR. No significant cross-reactivity or interference between Human KDR and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. KDR encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc.. Mutations of KDR are implicated in infantile capillary hemangiomas.
Notes	This Human Vascular endothelial cell growth factor receptor 2 (VEGFR-2/Flk-1) ELISA Kit employs a two-site sandwich ELISA to quantitate KDR in samples. An antibody specific for KDR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKDR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KDR is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KDR bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	3791

Applications	ELISA
Applications Notes	This Human Vascular endothelial cell growth factor receptor 2 (VEGFR-2/Flk-1) ELISA Kit employs a two-site sandwich ELISA to quantitate KDR in samples. An antibody specific for KDR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKDR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KDR is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KDR bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13704140	KTE61925	48 T	$339		Add to Cart
BHE13704140	KTE61925	96 T	$589		Add to Cart
BHE13704140	KTE61925	96 Tx5	$2629		Add to Cart
BHE13704140	KTE61925	96 Tx50	$24569		Add to Cart

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