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Home / ELISA Kit

Human Vascular Endothelial cell Growth Factor C (VEGF-C) ELISA Kit

SKU : BHE13702278

VEGF-C has been shown to exhibit angiogenic and lymphangiogenic actions. The VEGF family of growth factors and receptors is involved in the development and growth of the vascular endothelial system. Two of its family members, VEGF-C and VEGF-D, regulate the lymphatic endothelial cells via their receptor VEGFR, thus acting as mitogens for these cells. VEGF-C expression is associated with hematological malignancies. Like VEGF it acts as survival factor on leukemia. Together with the expression of their receptors, VEGF and VEGF-C result in the generation of autocrine loops that may support cancer cell survival and proliferation. Further VEGF-C expression has been shown in gastrointestinal tract malignancies where it correlates with lymphatic invasion, lymphnode metastasis and reduced survival.

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Mfr.No.	KTE60058
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Vascular Endothelial cell Growth Factor C (VEGF-C) ELISA Kit employs a two-site sandwich ELISA to quantitate VEGFC.
Alternative Names	VEGFC; Flt4-L; VRP; FLT4 ligand DHM; vascular endothelial growth factor-related protein
Detection Method	Colorimetric
Kit Components	Human Vascular Endothelial cell Growth Factor C microplate Human Vascular Endothelial cell Growth Factor C standard Human Vascular Endothelial cell Growth Factor C detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Vascular Endothelial cell Growth Factor C (VEGF-C) ELISA Kit has high sensitivity and excellent specificity for detection of Human VEGFC. No significant cross-reactivity or interference between Human VEGFC and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	VEGF-C has been shown to exhibit angiogenic and lymphangiogenic actions. The VEGF family of growth factors and receptors is involved in the development and growth of the vascular endothelial system. Two of its family members, VEGF-C and VEGF-D, regulate the lymphatic endothelial cells via their receptor VEGFR, thus acting as mitogens for these cells. VEGF-C expression is associated with hematological malignancies. Like VEGF it acts as survival factor on leukemia. Together with the expression of their receptors, VEGF and VEGF-C result in the generation of autocrine loops that may support cancer cell survival and proliferation. Further VEGF-C expression has been shown in gastrointestinal tract malignancies where it correlates with lymphatic invasion, lymphnode metastasis and reduced survival.
Notes	This Human Vascular Endothelial cell Growth Factor C (VEGF-C) ELISA Kit employs a two-site sandwich ELISA to quantitate VEGFC in samples. An antibody specific for VEGFC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyVEGFC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VEGFC is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VEGFC bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	7424

Applications	ELISA
Applications Notes	This Human Vascular Endothelial cell Growth Factor C (VEGF-C) ELISA Kit employs a two-site sandwich ELISA to quantitate VEGFC in samples. An antibody specific for VEGFC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyVEGFC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VEGFC is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VEGFC bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702278	KTE60058	48 T	$379		Add to Cart
BHE13702278	KTE60058	96 T	$719		Add to Cart
BHE13702278	KTE60058	96 Tx5	$3159		Add to Cart
BHE13702278	KTE60058	96 Tx50	$28079		Add to Cart

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