Human Vascular Endothelial Cell Growth Factor B

Mfr.No.

E0133Hu

Description

This sandwich kit is for the accurate quantitative detection of human Vascular Endothelial cell Growth Factor B (also known as VEGF-B) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Vascular endothelial growth factor B;VEGF B;VEGFB;VEGF-B;VEGF-related factor;VRF

Reactivity

Human

Detection Range

20-1200ng/L

Sample Type

Serum, Cell culture supernatants, Plasma

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

21602

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

10.11ng/L

Background

Growth factor for endothelial cells. VEGF-B167 binds heparin and neuropilin-1 whereas the binding to neuropilin-1 of VEGF-B186 is regulated by proteolysis.

Gene ID	7423
UniProt ID	P49765
Antigen	VEGFB

Research Area

Cardiovascular; Signal Transduction; Neuroscience; Cancer

Protocol

These standard curves of E0133Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
640ng/L	2.323	2.264	2.236
	2.204
320ng/L	1.309	1.369	1.342
	1.43
160ng/L	0.73	0.741	0.714
	0.753
80ng/L	0.298	0.358	0.331
	0.419
40ng/L	0.216	0.2	0.173
	0.185
0ng/L	0.036	0.028	0
	0.019

Precision

We measured random samples of E0133Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	271.7	12.02	4.4
Intra-Assay	2	18	358	12.73	3.6
Intra-Assay	3	18	108.2	5.71	5.3

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12101868	E0133Hu	96T	$458		Add to Cart
BHE12101868	E0133Hu	48T	$320		Add to Cart

Human Vascular Endothelial Cell Growth Factor B - VEGF-B ELISA Kit

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