Human Vascular Endothelial Cell Growth Factor A

Mfr.No.

E0050Hu

Description

This sandwich kit is for the accurate quantitative detection of human Vascular Endothelial cell Growth Factor A (also known as VEGF-A) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Vascular endothelial growth factor A;Vascular permeability factor;VEGF A;VEGFA;VEGF-A;VPF

Reactivity

Human

Detection Range

3-900ng/L

Sample Type

Serum, Cell culture supernatants, Plasma

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

27042

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

1.52ng/L

Background

Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. Binding to NRP1 receptor initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (By similarity).

Gene ID	7422
UniProt ID	P15692
Antigen	VEGFA

Research Area

Cancer

Protocol

These standard curves of E0050Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
480ng/L	2.628	2.533	2.472
	2.438
240ng/L	1.444	1.451	1.391
	1.458
120ng/L	0.825	0.771	0.711
	0.718
60ng/L	0.51	0.495	0.435
	0.48
30ng/L	0.246	0.254	0.193
	0.262
0ng/L	0.065	0.06	0
	0.056

Precision

We measured random samples of E0050Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	61.8	3.38	5.5
Intra-Assay	2	18	176.2	9.42	5.3
Intra-Assay	3	18	208.3	5.53	2.7

Storage	2-8°C

Reference	Bevacizumab Inhibits Angiogenic Cytokines in Head and Neck Squamous Cell Carcinoma: From Gene to the Protein Journal: Int J Hematol Oncol Stem Cell Res. 2018 Apr 1;12(2):136-141 Bevacizumab Inhibits Angiogenic Cytokines in Head and Neck Squamous Cell Carcinoma: From Gene to the Protein Journal: Int J Hematol Oncol Stem Cell Res. 2018 Apr 1;12(2):136-141.

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SKU	Supplier No.	Size	Your price	Quantity
BHE12101785	E0050Hu	96T	$458		Add to Cart
BHE12101785	E0050Hu	48T	$320		Add to Cart

Human Vascular Endothelial Cell Growth Factor A - VEGF-A ELISA Kit

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