Human Vascular Cell Adhesion Molecule 1

Mfr.No.

E0203Hu

Sensitivity

0.23ng/ml

Detection Range

0.5-200ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Vascuolar Cell Adhesion Molecule 1 (also known as VCAM-1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

CD antigen CD106;INCAM-100;Vascular cell adhesion protein 1;VCAM 1;V-CAM 1;VCAM1;VCAM-1

Molecular Weight

81276

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0203Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	14.3	1.03	7.2
Intra-Assay	2	18	39.5	2.75	7
Intra-Assay	3	18	68.2	3.32	4.9

Background

Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with integrin alpha-4/beta-1 (ITGA4/ITGB1) on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/ITGA4/ITGB1 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.

Protocol

These standard curves of E0203Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
120ng/ml	2.239	2.13	2.081
	2.021
60ng/ml	1.395	1.338	1.29
	1.282
30ng/ml	0.902	0.874	0.825
	0.845
15ng/ml	0.444	0.483	0.434
	0.521
7.5ng/ml	0.309	0.282	0.234
	0.255
0ng/ml	0.037	0.049	0
	0.061

Gene ID	7412
UniProt ID	P19320

Research Area	Cancer

Storage	2-8°C

Reference	The effect of coenzyme Q10 supplementation on inflammatory and endothelial dysfunction markers in overweight/obese polycystic ovary syndrome patients Journal: Gynecol Endocrinol.2.28.2021 Jan;37(1):26-30. Association between Vascular Endothelial Markers and Carotid Intima-Media Thickness in Children and Adolescents with Type 1 Diabetes Mellitus Journal: J Clin Diagn Res. 2017 Sep;11(9):SC01-SC05.

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SKU	Supplier No.	Size	Your price	Quantity
BHE12101923	E0203Hu	96T	$458		Add to Cart
BHE12101923	E0203Hu	48T	$320		Add to Cart

Human Vascular Cell Adhesion Molecule 1 - VCAM-1 ELISA Kit

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