Human Vascular Cell Adhesion Molecule 1

Mfr.No.

E0203Hu

Description

This sandwich kit is for the accurate quantitative detection of human Vascuolar Cell Adhesion Molecule 1 (also known as VCAM-1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

CD antigen CD106;INCAM-100;Vascular cell adhesion protein 1;VCAM 1;V-CAM 1;VCAM1;VCAM-1

Reactivity

Human

Detection Range

0.5-200ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

81276

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.23ng/ml

Background

Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with integrin alpha-4/beta-1 (ITGA4/ITGB1) on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/ITGA4/ITGB1 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.

Gene ID	7412
UniProt ID	P19320
Antigen	VCAM1

Research Area

Cancer

Protocol

These standard curves of E0203Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
120ng/ml	2.239	2.13	2.081
	2.021
60ng/ml	1.395	1.338	1.29
	1.282
30ng/ml	0.902	0.874	0.825
	0.845
15ng/ml	0.444	0.483	0.434
	0.521
7.5ng/ml	0.309	0.282	0.234
	0.255
0ng/ml	0.037	0.049	0
	0.061

Precision

We measured random samples of E0203Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	14.3	1.03	7.2
Intra-Assay	2	18	39.5	2.75	7
Intra-Assay	3	18	68.2	3.32	4.9

Storage	2-8°C

Reference	The effect of coenzyme Q10 supplementation on inflammatory and endothelial dysfunction markers in overweight/obese polycystic ovary syndrome patients Journal: Gynecol Endocrinol.2.28.2021 Jan;37(1):26-30. Association between Vascular Endothelial Markers and Carotid Intima-Media Thickness in Children and Adolescents with Type 1 Diabetes Mellitus Journal: J Clin Diagn Res. 2017 Sep;11(9):SC01-SC05.

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SKU	Supplier No.	Size	Your price	Quantity
BHE12101923	E0203Hu	96T	$458		Add to Cart
BHE12101923	E0203Hu	48T	$320		Add to Cart

Human Vascular Cell Adhesion Molecule 1 - VCAM-1 ELISA Kit

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