JavaScript is currently not supported/disabled by this browser. Please enable JavaScript for full functionality.

Call Us 1 - 866 - 986 - 9598
[email protected]

Sign Up

Login
- My Order
- Return Requests
- Track my Order

Home / ELISA Kit

Human Vacuolar protein sorting-associated protein 37A (VPS37A) ELISA Kit

SKU : BHE13702268

HCRP1 encodes a deduced 397-amino acid protein. Northern blot analysis showed ubiquitous expression of an approximately 2-kb HCRP1 transcript, with most abundant expression in liver. Western blot analysis showed reduced HCRP1 expression in 6 of 8 HCC tissues, but higher expression levels of HCRP1 in adjacent normal tissues in all 8 cases. Transient transfection of GFP-HCRP1 fusion constructs showed that HCRP1 is localized throughout the cell, but predominantly in the nucleus.Pull-down assays demonstrated that the interaction between HCRP1 and TSG101 occurs through the mod(r) domain. By size exclusion chromatography, HCRP1 cofractionated with TSG101 and VPS28. Depletion of TSG101 by siRNA treatment resulted in reduction in HCRP1 levels in HeLa cells.

Continuing the saving: $50 Off All ELISA Kits Offer Extended!

Contact us to order

Tel

+1 866.986.9598

Email

[email protected]

Get a Quote

Request More Infos

Abbkine Scientific Co., Ltd.

view supplier details

Order Now

Credit card payments now incur a 3% fee.

Contact us to order

Tel

+1 866.986.9598

Email

[email protected]

Get a Quote

Ask Merchant

Request More Infos

Sample Request

Mfr.No.	KTE60048
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Vacuolar protein sorting-associated protein 37A (VPS37A) ELISA Kit employs a two-site sandwich ELISA to quantitate VPS37A.
Alternative Names	VPS37A; FLJ32642; FLJ42616; HCRP1; PQBP2; hepatocellular carcinoma related protein 1; polyglutamine binding protein 2; vacuolar protein sorting 37A
Detection Method	Colorimetric
Kit Components	Human Vacuolar protein sorting-associated protein 37A microplate Human Vacuolar protein sorting-associated protein 37A standard Human Vacuolar protein sorting-associated protein 37A detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Vacuolar protein sorting-associated protein 37A (VPS37A) ELISA Kit has high sensitivity and excellent specificity for detection of Human VPS37A. No significant cross-reactivity or interference between Human VPS37A and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	HCRP1 encodes a deduced 397-amino acid protein. Northern blot analysis showed ubiquitous expression of an approximately 2-kb HCRP1 transcript, with most abundant expression in liver. Western blot analysis showed reduced HCRP1 expression in 6 of 8 HCC tissues, but higher expression levels of HCRP1 in adjacent normal tissues in all 8 cases. Transient transfection of GFP-HCRP1 fusion constructs showed that HCRP1 is localized throughout the cell, but predominantly in the nucleus.Pull-down assays demonstrated that the interaction between HCRP1 and TSG101 occurs through the mod(r) domain. By size exclusion chromatography, HCRP1 cofractionated with TSG101 and VPS28. Depletion of TSG101 by siRNA treatment resulted in reduction in HCRP1 levels in HeLa cells.
Notes	This Human Vacuolar protein sorting-associated protein 37A (VPS37A) ELISA Kit employs a two-site sandwich ELISA to quantitate VPS37A in samples. An antibody specific for VPS37A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyVPS37A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VPS37A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VPS37A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	137492

Applications	ELISA
Applications Notes	This Human Vacuolar protein sorting-associated protein 37A (VPS37A) ELISA Kit employs a two-site sandwich ELISA to quantitate VPS37A in samples. An antibody specific for VPS37A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyVPS37A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VPS37A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VPS37A bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

No Description Available.

Have you used this product?

Rate it now..!!

Login to Review

SKU	Supplier No.	Size	Your price	Quantity
BHE13702268	KTE60048	48 T	$339		Add to Cart
BHE13702268	KTE60048	96 T	$589		Add to Cart
BHE13702268	KTE60048	96 Tx5	$2629		Add to Cart
BHE13702268	KTE60048	96 Tx50	$24569		Add to Cart

ebiohippo.com provides biomedical researchers with a one-stop informatic shopping experience by transforming biotechnology products’ data into a ready-to-analyze state. We collect and unify suppliers’ data on a single platform to reduce the time of searching, uncertainty, and cost of scientific experiments.

© 2024 Biohippo. All rights reserved.