Human V-Set Domain Containing T-cell Activation Inhibitor 1

Mfr.No.

E2620Hu

Description

This sandwich kit is for the accurate quantitative detection of human V-set Vomain-containing T-cell Activation Inhibitor 1 (also known as VTCN1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

B7 homolog 4;B7h.5;B7-H4;Immune costimulatory protein B7-H4;Protein B7S1;T-cell costimulatory molecule B7x;V-set domain-containing T-cell activation inhibitor 1;VTCN 1;VTCN1;

Reactivity

Human

Detection Range

0.05-20ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

30878

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.021ng/ml

Background

Negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, plays an important role, together with regulatory T-cells (Treg), in the suppression of tumor-associated antigen-specific T-cell immunity. Involved in promoting epithelial cell transformation.

Gene ID	79679
UniProt ID	Q7Z7D3
Antigen	VTCN1

Research Area

Immunology

Protocol

These standard curves of E2620Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12.8ng/ml	2.135	2.052	2.01
	1.969
6.4ng/ml	1.117	1.07	1.028
	1.024
3.2ng/ml	0.578	0.569	0.527
	0.56
1.6ng/ml	0.232	0.232	0.19
	0.232
0.8ng/ml	0.148	0.116	0.073
	0.084
0ng/ml	0.03	0.042	0
	0.054

Precision

We measured random samples of E2620Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	6.8	0.28	4.1
Intra-Assay	2	18	9.7	0.3	3.1
Intra-Assay	3	18	3.8	0.2	5.2

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104158	E2620Hu	48T	$320		Add to Cart
BHE12104158	E2620Hu	96T	$458		Add to Cart

Human V-Set Domain Containing T-cell Activation Inhibitor 1 - VTCN1 ELISA Kit

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