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Home / ELISA Kit

Human UV excision repair protein RAD23 homolog B (RAD23B) ELISA Kit

SKU : BHE13703201

RAD23B is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in the nucleotide excision repair (NER). This protein was found to be a component of the protein complex that specifically complements the NER defect of xeroderma pigmentosum group C (XP-c) cell extracts in vitro.
This protein was also shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, and thus this protein may be involved in the ubiquitin mediated proteolytic pathway in cells.

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Mfr.No.	KTE60985
Description	This Human UV excision repair protein RAD23 homolog B (RAD23B) ELISA Kit employs a two-site sandwich ELISA to quantitate RAD23B.
Alternative Names	RAD23B; RP11-131A5.1; HHR23B; HR23B; P58; RAD23; yeast homolog of; B; UV excision repair protein RAD23 homolog B; XP-C repair complementing complex 58 kDa; XP-C repair complementing protein
Reactivity	Human
Detection Method	Colorimetric
Sample Type	Other biological fluids, Cell culture supernatants, Serum, Plasma
Assay Type	Sandwich (quantitative)
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Components	Human UV excision repair protein RAD23 homolog B microplate Human UV excision repair protein RAD23 homolog B standard Human UV excision repair protein RAD23 homolog B detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human UV excision repair protein RAD23 homolog B (RAD23B) ELISA Kit has high sensitivity and excellent specificity for detection of Human RAD23B. No significant cross-reactivity or interference between Human RAD23B and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	RAD23B is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in the nucleotide excision repair (NER). This protein was found to be a component of the protein complex that specifically complements the NER defect of xeroderma pigmentosum group C (XP-c) cell extracts in vitro. This protein was also shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, and thus this protein may be involved in the ubiquitin mediated proteolytic pathway in cells.
Notes	This Human UV excision repair protein RAD23 homolog B (RAD23B) ELISA Kit employs a two-site sandwich ELISA to quantitate RAD23B in samples. An antibody specific for RAD23B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRAD23B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RAD23B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RAD23B bound in the initial step. The color development is stopped and the intensity of the color is measured.

Gene ID	5887
Antigen	Rad23B
Tag	RAD23B
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Human UV excision repair protein RAD23 homolog B (RAD23B) ELISA Kit employs a two-site sandwich ELISA to quantitate RAD23B in samples. An antibody specific for RAD23B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRAD23B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RAD23B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RAD23B bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703201	KTE60985	48 T	$339		Add to Cart
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