Matrices listed below were spiked with certain level of USP22 and the recovery rates were calculated by comparing the measured value to the expected amount of USP22 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-103
97
EDTA plasma(n=5)
92-105
99
heparin plasma(n=5)
86-101
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of USP22 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
86-102%
88-103%
88-104%
EDTA plasma(n=5)
82-94%
86-99%
90-101%
heparin plasma(n=5)
92-100%
80-94%
83-97%
Sensitivity
< 0.094ng/ml
Notes
For Research Use Only
UniProt ID
Q9UPT9
Applications
ELISA
Applications Notes
USP22ELISA Kit allows for the in vitro quantitative determination of USP22 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human USP22(Ubiquitin thioesterase 22)ELISA Kit