Matrices listed below were spiked with certain level of USP14 and the recovery rates were calculated by comparing the measured value to the expected amount of USP14 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-103
98
EDTA plasma(n=5)
92-100
96
heparin plasma(n=5)
85-103
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of USP14 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
85-98%
87-104%
89-96%
EDTA plasma(n=5)
90-99%
83-98%
84-101%
heparin plasma(n=5)
81-100%
84-94%
81-99%
Sensitivity
< 18.75pg/ml
Notes
For Research Use Only
UniProt ID
P54578
Applications
ELISA
Applications Notes
USP14ELISA Kit allows for the in vitro quantitative determination of USP14 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human USP14(Ubiquitin carboxyl-terminal hydrolase 14)ELISA Kit