Matrices listed below were spiked with certain level of USP1 and the recovery rates were calculated by comparing the measured value to the expected amount of USP1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
94-102
98
EDTA plasma(n=5)
88-101
95
heparin plasma(n=5)
88-101
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of USP1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
85-97%
88-103%
86-91%
EDTA plasma(n=5)
86-100%
87-101%
82-100%
heparin plasma(n=5)
80-99%
80-100%
81-95%
Sensitivity
< 0.094ng/ml
Notes
For Research Use Only
UniProt ID
O94782
Applications
ELISA
Applications Notes
USP1 ELISA Kit allows for the in vitro quantitative determination of USP1 concentrations in cell lysates, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human USP1(Ubiquitin carboxyl-terminal hydrolase 1) ELISA Kit