Human Urocortin - UCN LISA KIT

Mfr.No.

E1406Hu

Description

This sandwich kit is for the accurate quantitative detection of human Adipocyte Fatty Acid-binding Protein (also known as A-FABP) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

UCN;Urocortin

Reactivity

Human

Detection Range

0.05-10ng/ml

Sample Type

Serum, Cell culture supernatants, Plasma

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

13458

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.01ng/ml

Background

Acts in vitro to stimulate the secretion of adrenocorticotropic hormone (ACTH) (PubMed: 8612563). Binds with high affinity to CRF receptor types 1, 2-alpha, and 2-beta (PubMed: 8612563). Plays a role in the establishment of normal hearing thresholds (By similarity). Reduces food intake and regulates ghrelin levels in gastric body and plasma (By similarity).

Gene ID	7349
UniProt ID	P55089
Antigen	UCN

Research Area

Others

Protocol

These standard curves of E1406Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
6.4ng/ml	2.082	2.103	2.067
	2.124
3.2ng/ml	1.736	1.635	1.598
	1.534
1.6ng/ml	1.072	1.019	0.983
	0.965
0.8ng/ml	0.68	0.68	0.644
	0.679
0.4ng/ml	0.48	0.449	0.412
	0.418
0ng/ml	0.029	0.036	0
	0.044

Precision

We measured random samples of E1406Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	2.5	0.13	5.1
Intra-Assay	2	18	1	0.06	6.2
Intra-Assay	3	18	0.5	0.03	6

Storage	2-8°C

Reference	Effects of administration of omega-3 fatty acids with or without vitamin E supplementation on adiponectin gene expression in PBMCs and serum adiponectin and adipocyte fatty acid-binding protein levels in male patients with CAD Journal: Anatol J Cardiol. 2015;15(12):981-9.

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SKU	Supplier No.	Size	Your price	Quantity
BHE12103034	E1406Hu	48T	$320		Add to Cart
BHE12103034	E1406Hu	96T	$458		Add to Cart

Human Urocortin - UCN LISA KIT

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