Human Uridine Phosphorylase 1

Mfr.No.

E0819Hu

Alternative Names

UPase 1;UPP 1;UPP1;UrdPase 1;Uridine phosphorylase 1

Reactivity

Human

Detection Range

0.05-15ng/mL

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

33934

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.023ng/mL

Background

Catalyzes the reversible phosphorylytic cleavage of uridine and deoxyuridine to uracil and ribose- or deoxyribose-1-phosphate (PubMed: 7488099). The produced molecules are then utilized as carbon and energy sources or in the rescue of pyrimidine bases for nucleotide synthesis.

Gene ID	7378
UniProt ID	Q16831
Antigen	UPP1

Research Area

Others

Protocol

These standard curves of E0819Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
8ng/mL	2.054	2.134	2.09
	2.214
4ng/mL	1.166	1.159	1.114
	1.151
2ng/mL	0.81	0.798	0.754
	0.785
1ng/mL	0.411	0.41	0.366
	0.409
0.5ng/mL	0.214	0.236	0.192
	0.258
0ng/mL	0.037	0.044	0
	0.051

Precision

We measured random samples of E0819Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	2.4	0.15	6.2
Intra-Assay	2	18	6.6	0.23	3.5
Intra-Assay	3	18	1.1	0.08	7.3

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12102498	E0819Hu	48T	$320		Add to Cart
BHE12102498	E0819Hu	96T	$458		Add to Cart

Human Uridine Phosphorylase 1 - UPP1 ELISA Kit

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