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Home / ELISA Kit

Human Uracil phosphoribosyltransferase homolog (UPRT) ELISA Kit

SKU : BHE13702304

Uqcc, called Bfzb, and identified its human homolog by database analysis. The deduced human and mouse proteins have a single transmembrane domain and share 94% amino acid identity. In situ hybridization of mouse embryos detected Bfzb predominantly in the developing nervous system, with highest expression in proliferating neuroepithelia of brain and neural tube, ganglia of cranial nerves V, VII, VIII, IX, and X, and dorsal root ganglia.
Transcripts were also detected in developing eye and brown fat. In adult mouse brain, strong expression was restricted to olfactory bulb, hippocampus, and piriform cortex and Purkinje cells of cerebellum. The Bfzb protein localized to cytoplasmic vesicular structures.

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Mfr.No.	KTE60085
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Uracil phosphoribosyltransferase homolog (UPRT) ELISA Kit employs a two-site sandwich ELISA to quantitate UPRT.
Alternative Names	UPRT; RP11-311P8.3; DKFZp781E1243; FUR1; MGC23937; UPP; uracil phosphoribosyltransferase (FUR1) homolog
Detection Method	Colorimetric
Kit Components	Human Uracil phosphoribosyltransferase homolog microplate Human Uracil phosphoribosyltransferase homolog standard Human Uracil phosphoribosyltransferase homolog detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Uracil phosphoribosyltransferase homolog (UPRT) ELISA Kit has high sensitivity and excellent specificity for detection of Human UPRT. No significant cross-reactivity or interference between Human UPRT and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Uqcc, called Bfzb, and identified its human homolog by database analysis. The deduced human and mouse proteins have a single transmembrane domain and share 94% amino acid identity. In situ hybridization of mouse embryos detected Bfzb predominantly in the developing nervous system, with highest expression in proliferating neuroepithelia of brain and neural tube, ganglia of cranial nerves V, VII, VIII, IX, and X, and dorsal root ganglia. Transcripts were also detected in developing eye and brown fat. In adult mouse brain, strong expression was restricted to olfactory bulb, hippocampus, and piriform cortex and Purkinje cells of cerebellum. The Bfzb protein localized to cytoplasmic vesicular structures.
Notes	This Human Uracil phosphoribosyltransferase homolog (UPRT) ELISA Kit employs a two-site sandwich ELISA to quantitate UPRT in samples. An antibody specific for UPRT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyUPRT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UPRT is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UPRT bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	139596

Applications	ELISA
Applications Notes	This Human Uracil phosphoribosyltransferase homolog (UPRT) ELISA Kit employs a two-site sandwich ELISA to quantitate UPRT in samples. An antibody specific for UPRT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyUPRT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UPRT is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UPRT bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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