Matrices listed below were spiked with certain level of UNC13A/Munc13-1 and the recovery rates were calculated by comparing the measured value to the expected amount of UNC13A/Munc13-1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
96-104
99
EDTA plasma(n=5)
98-104
100
heparin plasma(n=5)
95-105
99
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of UNC13A/Munc13-1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
91-103%
90-103%
92-104%
EDTA plasma(n=5)
93-100%
89-101%
92-101%
heparin plasma(n=5)
92-99%
91-98%
90-97%
Sensitivity
< 9.375pg/ml
Notes
For Research Use Only
UniProt ID
Q9UPW8
Applications
ELISA
Applications Notes
UNC13A/Munc13-1 ELISA Kit allows for the in vitro quantitative determination of UNC13A/Munc13-1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human UNC13A/Munc13-1(Protein unc-13 homolog A) ELISA Kit