Human ULBP2 ELISA Kit PicoKine®

Mfr.No.

EK1593

Sensitivity

<12 pg/ml

Detection Range

62.5 pg/ml - 4,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	62.5	125	250	500	1000	2000	4000
O.D.	0.009	0.051	0.100	0.183	0.327	0.599	1.131	1.865

Description

Human ULBP2 ELISA Kit PicoKine® (96 Tests). Quantitate Human ULBP2 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 12pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

NKG2D ligand 2;N2DL-2;NKG2DL2;ALCAN-alpha;Retinoic acid early transcript 1H;UL16-binding protein 2;ULBP2;N2DL2, RAET1H;UNQ463/PRO791;

Molecular Weight

27368 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: G26-S216

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human ULBP2

Subcellular Localization

Cell membrane; Lipid-anchor, GPI-anchor. Secreted.

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	184	594	1005	181	539	1058
Standard deviation	11.77	46.92	46.23	11.76	49.58	58.19
CV (%)	6.4%	7.9%	4.6%	6.5%	9.2%	5.5%

Protein Function

Ligand for the KLRK1/NKG2D receptor, together with at least ULBP1 and ULBP3. ULBPs activate multiple signaling pathways in primary NK cells, resulting in the production of cytokines and chemokines. Binding of ULBPs ligands to KLRK1/NKG2D induces calcium mobilization and activation of the JAK2, STAT5, ERK and PI3K kinase/Akt signal transduction pathway. In CMV infected cells, interacts with soluble CMV glycoprotein UL16. The interaction with UL16 blocked the interaction with the KLRK1/NKG2D receptor, providing a mechanism by which CMV infected cells might escape the immune system. UL16 also causes ULBP2 to be retained in the ER and cis-Golgi apparatus so that it does not reach the cell surface. .

Background

UL16 binding protein 2 (ULBP2) is a cell surface glycoprotein encoded by ULBP2 gene located on the chromosome 6. This gene encodes a major histocompatibility complex (MHC) class I-related molecule that binds to the NKG2D receptor on natural killer (NK) cells to trigger release of multiple cytokines and chemokines that in tu contribute to the recruitment and activation of NK cells. The encoded protein undergoes further processing to generate the mature protein that is either anchored to membrane via a glycosylphosphatidylinositol moiety, or secreted. Many malignant cells secrete the encoded protein to evade immunosurveillance by NK cells.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	184	184	193	206	191	9.01	4.7%
Sample 2	594	557	578	574	575	13.16	2.2%
Sample 3	1005	1175	1073	1093	1086	60.61	5.5%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	80328
Gene Name	ULBP2
UniProt ID	Q9BZM5
Protein Name	NKG2D ligand 2
Tissue Specificity	Expressed in various types of cancer cell lines and in the fetus, but not in normal tissues. .

Applications	ELISA
Research Area	Immunology,Innate Immunity,NK Cells,TLR Signaling

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001274	EK1593	96 wells/kit	$499		Add to Cart

Human ULBP2 ELISA Kit PicoKine®

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