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Home / ELISA Kit

Human UDP-glucuronic acid decarboxylase 1 (UXS1) ELISA Kit

SKU : BHE13702295

UDP-glucuronate decarboxylase (UGD; EC 4.1.1.35) catalyzes the formation of UDP-xylose from UDP-glucuronate. UDP-xylose is then used to initiate glycosaminoglycan biosynthesis on the core protein of proteoglycans.
The topology of the deduced 420-amino acid protein was consistent with a type II transmembrane protein. Northern blot analysis of rat tissues detected a single UGD transcript with highest levels in heart, brain, and testes. Substantial levels were also detected in kidney, liver and lung, and lower levels in spleen and skeletal muscle. Western blot analysis demonstrated highest levels of the protein in kidney, liver, and brain, with negligible staining in heart, spleen, skeletal muscle, lung, and testes. Subcellular studies and histochemistry localized the protein to the perinuclear Golgi.

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Mfr.No.	KTE60076
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human UDP-glucuronic acid decarboxylase 1 (UXS1) ELISA Kit employs a two-site sandwich ELISA to quantitate UXS1.
Alternative Names	UXS1; FLJ23591; SDR6E1; UGD; short chain dehydrogenase/reductase family 6E; member 12
Detection Method	Colorimetric
Kit Components	Human UDP-glucuronic acid decarboxylase 1 microplate Human UDP-glucuronic acid decarboxylase 1 standard Human UDP-glucuronic acid decarboxylase 1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human UDP-glucuronic acid decarboxylase 1 (UXS1) ELISA Kit has high sensitivity and excellent specificity for detection of Human UXS1. No significant cross-reactivity or interference between Human UXS1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	UDP-glucuronate decarboxylase (UGD; EC 4.1.1.35) catalyzes the formation of UDP-xylose from UDP-glucuronate. UDP-xylose is then used to initiate glycosaminoglycan biosynthesis on the core protein of proteoglycans. The topology of the deduced 420-amino acid protein was consistent with a type II transmembrane protein. Northern blot analysis of rat tissues detected a single UGD transcript with highest levels in heart, brain, and testes. Substantial levels were also detected in kidney, liver and lung, and lower levels in spleen and skeletal muscle. Western blot analysis demonstrated highest levels of the protein in kidney, liver, and brain, with negligible staining in heart, spleen, skeletal muscle, lung, and testes. Subcellular studies and histochemistry localized the protein to the perinuclear Golgi.
Notes	This Human UDP-glucuronic acid decarboxylase 1 (UXS1) ELISA Kit employs a two-site sandwich ELISA to quantitate UXS1 in samples. An antibody specific for UXS1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyUXS1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UXS1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UXS1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	80146

Applications	ELISA
Applications Notes	This Human UDP-glucuronic acid decarboxylase 1 (UXS1) ELISA Kit employs a two-site sandwich ELISA to quantitate UXS1 in samples. An antibody specific for UXS1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyUXS1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UXS1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UXS1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702295	KTE60076	48 T	$339		Add to Cart
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BHE13702295	KTE60076	96 Tx50	$24569		Add to Cart

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