Matrices listed below were spiked with certain level of UCN2 and the recovery rates were calculated by comparing the measured value to the expected amount of UCN2 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
95-105
99
EDTA plasma(n=5)
88-100
93
heparin plasma(n=5)
88-103
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of UCN2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
UCN2 ELISA Kit allows for the in vitro quantitative determination of UCN2 concentrations in serum, plasma, tissue homogenates and other biological fluids.