Matrices listed below were spiked with certain level of UCK2 and the recovery rates were calculated by comparing the measured value to the expected amount of UCK2 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
82-104
95
EDTA plasma(n=5)
85-105
93
heparin plasma(n=5)
83-105
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of UCK2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
82-104%
89-100%
93-105%
EDTA plasma(n=5)
91-98%
91-97%
90-100%
heparin plasma(n=5)
90-98%
90-99%
91-100%
Sensitivity
< 9.375pg/ml
Notes
For Research Use Only
UniProt ID
Q9BZX2
Applications
ELISA
Applications Notes
UCK2 ELISA Kit allows for the in vitro quantitative determination of UCK2 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human UCK2(Uridine-cytidine kinase 2) ELISA Kit