Human Ubiquitin Specific Peptidase 8

Mfr.No.

E2338Hu

Sensitivity

0.021ng/ml

Detection Range

0.05-15ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

DEN1;Deneddylase-1;NEDD8-specific protease 1;NEDP1;Protease, cysteine 2;PRSC2;Sentrin/SUMO-specific protease SENP8;Sentrin-specific protease 8;USP8;

Molecular Weight

24107

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E2338Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	5.29	0.19	3.62
Intra-Assay	2	18	1.82	0.1	5.61
Intra-Assay	3	18	3.25	0.15	4.62

Background

Protease that catalyzes two essential functions in the NEDD8 pathway: processing of full-length NEDD8 to its mature form and deconjugation of NEDD8 from targeted proteins such as cullins or p53.

Protocol

These standard curves of E2338Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
8ng/ml	2.135	2.212	2.182
	2.289
4ng/ml	1.435	1.391	1.361
	1.347
2ng/ml	0.648	0.652	0.622
	0.657
1ng/ml	0.318	0.381	0.35
	0.443
0.5ng/ml	0.215	0.196	0.166
	0.177
0ng/ml	0.045	0.03	0
	0.015

Gene ID	123228
UniProt ID	Q96LD8

Research Area	Cell Biology

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12103876	E2338Hu	48T	$320		Add to Cart
BHE12103876	E2338Hu	96T	$458		Add to Cart

Human Ubiquitin Specific Peptidase 8 - USP8 ELISA Kit

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