Human ubiquitin-like modifier (ISG15) ELISA kit

Human ubiquitin-like modifier (ISG15) ELISA kit

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Mfr.No.

CSB-E12075h

Alternative Names

ISG15 ubiquitin-like modifier, G1P2, IFI15, UCRP, interferon, alpha-inducible protein (clone IFI-15K), interferon-induced 17-kDa/15-kDa protein, interferon-stimulated protein, 15 kDa, ubiquitin cross-reactive protein

Reactivity

Human

Detection Range

0.78 ng/mL-50 ng/mL

Sample Type

Tissue homogenates, Serum, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of rat Bcl2-L-1/Bcl-X spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	88-94
EDTA plasma (n=4)	99	90-102

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat Bcl2-L-1/Bcl-X in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-95
1:2	Average %	102
	Range %	97-104
1:4	Average %	91
	Range %	85-95
1:8	Average %	97
	Range %	91-101

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
3000	2.106	2.008	2.057	1.928
1500	1.416	1.376	1.396	1.267
750	0.928	0.965	0.947	0.818
375	0.596	0.605	0.601	0.472
187.5	0.364	0.353	0.359	0.230
93.75	0.293	0.284	0.289	0.160
46.88	0.215	0.207	0.211	0.082
0	0.130	0.128	0.129

Species

Homo sapiens

Sensitivity

0.195 ng/mL

Limit Of Detection

450 nm

UniProt ID	P05161
Antigen	ISG15 ubiquitin-like modifier

Applications

ELISA

Research Area

ISG15

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10504783	CSB-E12075h	96T	$835	Add to Cart
BHE10504783	CSB-E12075h	5x96T	$4008	Add to Cart
BHE10504783	CSB-E12075h	10x96T	$7932.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.