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Home / ELISA Kit

Human Ubiquitin-conjugating enzyme E2 C (UBE2C) ELISA Kit

SKU : BHE13702321

UBE2CBP bound UBE2C and that the amino acid sequence between 235 and 257 was necessary for this binding. UBE2CBP showed a self-ubiquitinylation activity in a HECT-like sequence-dependent manner. The C-terminal half (amino acids 188-389) showed stronger activity than the full-length UBE2CBP. In addition, the C-terminal half of UBE2CBP was able to bind cyclin B and ubiquitinylate cyclin B in vitro.
UBE2CBP gene contains 10 exons and spans approximately 176 kb.The C terminus of UBE2CBP shows weak homology to the HECT domain, which is conserved in one of the ubiquitin ligase (E3) families. Members of this family accept the ubiquitin moiety from E2 and transfer it to the target protein.

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Mfr.No.	KTE60102
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Ubiquitin-conjugating enzyme E2 C (UBE2C) ELISA Kit employs a two-site sandwich ELISA to quantitate UBE2C.
Alternative Names	UBE2C; RP3-447F3.1; UBCH10; dJ447F3.2; cyclin-selective ubiquitin carrier protein; mitotic-specific ubiquitin-conjugating enzyme; ubiquitin carrier protein E2-C; ubiquitin-protein ligase C
Detection Method	Colorimetric
Kit Components	Human Ubiquitin-conjugating enzyme E2 C microplate Human Ubiquitin-conjugating enzyme E2 C standard Human Ubiquitin-conjugating enzyme E2 C detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Ubiquitin-conjugating enzyme E2 C (UBE2C) ELISA Kit has high sensitivity and excellent specificity for detection of Human UBE2C. No significant cross-reactivity or interference between Human UBE2C and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	UBE2CBP bound UBE2C and that the amino acid sequence between 235 and 257 was necessary for this binding. UBE2CBP showed a self-ubiquitinylation activity in a HECT-like sequence-dependent manner. The C-terminal half (amino acids 188-389) showed stronger activity than the full-length UBE2CBP. In addition, the C-terminal half of UBE2CBP was able to bind cyclin B and ubiquitinylate cyclin B in vitro. UBE2CBP gene contains 10 exons and spans approximately 176 kb.The C terminus of UBE2CBP shows weak homology to the HECT domain, which is conserved in one of the ubiquitin ligase (E3) families. Members of this family accept the ubiquitin moiety from E2 and transfer it to the target protein.
Notes	This Human Ubiquitin-conjugating enzyme E2 C (UBE2C) ELISA Kit employs a two-site sandwich ELISA to quantitate UBE2C in samples. An antibody specific for UBE2C has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyUBE2C present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UBE2C is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UBE2C bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	11065

Applications	ELISA
Applications Notes	This Human Ubiquitin-conjugating enzyme E2 C (UBE2C) ELISA Kit employs a two-site sandwich ELISA to quantitate UBE2C in samples. An antibody specific for UBE2C has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyUBE2C present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UBE2C is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UBE2C bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702321	KTE60102	48 T	$339		Add to Cart
BHE13702321	KTE60102	96 T	$589		Add to Cart
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