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Home / ELISA Kit

Human Ubiquitin carboxyl-terminal hydrolase 14 (USP14) ELISA Kit

SKU : BHE13702300

Ubiquitin carboxyl-terminal hydrolase 14 is a member of the ubiquitin-specific processing (UBP) family of proteases that is a deubiquitinating enzyme (DUB) with His and Cys domains.
This protein is located in the cytoplasm and cleaves the ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins. Mice with a mutation that results in reduced expression of the ortholog of this protein are retarded for growth, develop severe tremors by 2 to 3 weeks of age followed by hindlimb paralysis and death by 6 to 10 weeks of age. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

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Mfr.No.	KTE60081
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Ubiquitin carboxyl-terminal hydrolase 14 (USP14) ELISA Kit employs a two-site sandwich ELISA to quantitate USP14.
Alternative Names	USP14; TGT; deubiquitinating enzyme 14; tRNA-guanine transglycosylase; 60-kD subunit; ubiquitin carboxyl-terminal hydrolase 14; ubiquitin specific protease 14; ubiquitin specific protease 14 (tRNA-guanine tran
Detection Method	Colorimetric
Kit Components	Human Ubiquitin carboxyl-terminal hydrolase 14 microplate Human Ubiquitin carboxyl-terminal hydrolase 14 standard Human Ubiquitin carboxyl-terminal hydrolase 14 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Ubiquitin carboxyl-terminal hydrolase 14 (USP14) ELISA Kit has high sensitivity and excellent specificity for detection of Human USP14. No significant cross-reactivity or interference between Human USP14 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Ubiquitin carboxyl-terminal hydrolase 14 is a member of the ubiquitin-specific processing (UBP) family of proteases that is a deubiquitinating enzyme (DUB) with His and Cys domains. This protein is located in the cytoplasm and cleaves the ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins. Mice with a mutation that results in reduced expression of the ortholog of this protein are retarded for growth, develop severe tremors by 2 to 3 weeks of age followed by hindlimb paralysis and death by 6 to 10 weeks of age. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Notes	This Human Ubiquitin carboxyl-terminal hydrolase 14 (USP14) ELISA Kit employs a two-site sandwich ELISA to quantitate USP14 in samples. An antibody specific for USP14 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyUSP14 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for USP14 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of USP14 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	9097

Applications	ELISA
Applications Notes	This Human Ubiquitin carboxyl-terminal hydrolase 14 (USP14) ELISA Kit employs a two-site sandwich ELISA to quantitate USP14 in samples. An antibody specific for USP14 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyUSP14 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for USP14 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of USP14 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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