Human Ubiquitin-associated and Sh3 Domain-containing Protein A

Mfr.No.

E6375Hu

Sensitivity

5.14ng/L

Detection Range

10-2000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Cbl-interacting protein 4;CLIP4;STS-2;Suppressor of T-cell receptor signaling 2;T-cell ubiquitin ligand 1;TULA;TULA-1;UBASH3A;Ubiquitin-associated and SH3 domain-containing protein A;

Molecular Weight

74123

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E6375Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	382.3	18.11	4.74
Intra-Assay	2	18	935.96	29.52	3.15
Intra-Assay	3	18	237.2	15.49	6.53

Background

Interferes with CBL-mediated down-regulation and degradation of receptor-type tyrosine kinases. Promotes accumulation of activated target receptors, such as T-cell receptors, EGFR and PDGFRB, on the cell surface. Exhibits negligigle protein tyrosine phosphatase activity at neutral pH. May act as a dominant-negative regulator of UBASH3B-dependent dephosphorylation. May inhibit dynamin-dependent endocytic pathways by functionally sequestering dynamin via its SH3 domain.

Protocol

These standard curves of E6375Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200ng/L	2.091	2.181	2.146
	2.271
600ng/L	1.337	1.258	1.223
	1.179
300ng/L	0.634	0.715	0.68
	0.796
150ng/L	0.392	0.44	0.406
	0.489
75ng/L	0.258	0.218	0.183
	0.177
0ng/L	0.024	0.035	0
	0.045

Gene ID	53347
UniProt ID	P57075

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12107737	E6375Hu	48T	$320		Add to Cart
BHE12107737	E6375Hu	96T	$458		Add to Cart

Human Ubiquitin-associated and Sh3 Domain-containing Protein A - UBASH3A ELISA Kit

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