Human Ubiquitin-associated and Sh3 Domain-containing Protein A

Mfr.No.

E6375Hu

Alternative Names

Cbl-interacting protein 4;CLIP4;STS-2;Suppressor of T-cell receptor signaling 2;T-cell ubiquitin ligand 1;TULA;TULA-1;UBASH3A;Ubiquitin-associated and SH3 domain-containing protein A;

Reactivity

Human

Detection Range

10-2000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

74123

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

5.14ng/L

Background

Interferes with CBL-mediated down-regulation and degradation of receptor-type tyrosine kinases. Promotes accumulation of activated target receptors, such as T-cell receptors, EGFR and PDGFRB, on the cell surface. Exhibits negligigle protein tyrosine phosphatase activity at neutral pH. May act as a dominant-negative regulator of UBASH3B-dependent dephosphorylation. May inhibit dynamin-dependent endocytic pathways by functionally sequestering dynamin via its SH3 domain.

Gene ID	53347
UniProt ID	P57075
Antigen	UBASH3A

Research Area

Others

Protocol

These standard curves of E6375Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200ng/L	2.091	2.181	2.146
	2.271
600ng/L	1.337	1.258	1.223
	1.179
300ng/L	0.634	0.715	0.68
	0.796
150ng/L	0.392	0.44	0.406
	0.489
75ng/L	0.258	0.218	0.183
	0.177
0ng/L	0.024	0.035	0
	0.045

Precision

We measured random samples of E6375Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	382.3	18.11	4.74
Intra-Assay	2	18	935.96	29.52	3.15
Intra-Assay	3	18	237.2	15.49	6.53

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12107737	E6375Hu	48T	$320		Add to Cart
BHE12107737	E6375Hu	96T	$458		Add to Cart

Human Ubiquitin-associated and Sh3 Domain-containing Protein A - UBASH3A ELISA Kit

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