Matrices listed below were spiked with certain level of UBC and the recovery rates were calculated by comparing the measured value to the expected amount of UBC in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-105
94
EDTA plasma(n=5)
84-101
94
heparin plasma(n=5)
80-103
93
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of UBC and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
83-98%
85-99%
83-104%
EDTA plasma(n=5)
92-101%
91-101%
90-99%
heparin plasma(n=5)
90-100%
90-98%
90-98%
Sensitivity
< 0.188ng/ml
Notes
For Research Use Only
UniProt ID
P0CG48
Applications
ELISA
Applications Notes
UBC ELISA Kit allows for the in vitro quantitative determination of UBC concentrations in serum, plasma, tissue homogenates and other biological fluids.