Human U5 Small Nuclear Ribonucleoprotein 200 kDa Helicase

Mfr.No.

E6370Hu

Alternative Names

Activating signal cointegrator 1 complex subunit 3-like 1;ASCC3L1;BRR2;BRR2 homolog;HELIC2;RP33;SNRNP200;U5 small nuclear ribonucleoprotein 200 kDa helicase;U5 snRNP-specific 200 kDa protein;U5-200KD

Reactivity

Human

Detection Range

15-3000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

244508

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

7.46ng/L

Background

Plays role in pre-mRNA splicing as core component of precatalytic, catalytic and postcatalytic spliceosomal complexes (PubMed:28502770, PubMed:28781166, PubMed:29361316, PubMed:30315277, PubMed:29360106, PubMed:29301961, PubMed:30728453, PubMed:30705154). Involved in spliceosome assembly, activation and disassembly. Mediates changes in the dynamic network of RNA-RNA interactions in the spliceosome. Catalyzes the ATP-dependent unwinding of U4/U6 RNA duplices, an essential step in the assembly of a catalytically active spliceosome.

Gene ID	23020
UniProt ID	O75643
Antigen	SNRNP200

Research Area

Others

Protocol

These standard curves of E6370Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.072	2.08	2.038
	2.087
800ng/L	1.38	1.445	1.403
	1.509
400ng/L	0.765	0.751	0.709
	0.738
200ng/L	0.451	0.382	0.341
	0.314
100ng/L	0.175	0.159	0.117
	0.143
0ng/L	0.052	0.042	0
	0.032

Precision

We measured random samples of E6370Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	609.83	31.61	5.18
Intra-Assay	2	18	1175.58	58.48	4.97
Intra-Assay	3	18	193.77	8.23	4.25

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12107732	E6370Hu	48T	$320		Add to Cart
BHE12107732	E6370Hu	96T	$458		Add to Cart

Human U5 Small Nuclear Ribonucleoprotein 200 kDa Helicase - SNRNP200 ELISA Kit

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